添加秸秆和黑炭对水稻土碳氮转化及土壤微生物代谢图谱的影响

doi:10.3969/j.issn.10017216.2013.01.014

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (1): 97-104.DOI: 10.3969/j.issn.10017216.2013.01.014

添加秸秆和黑炭对水稻土碳氮转化及土壤微生物代谢图谱的影响

王娟^1，2,张丽君¹,姚槐应^1，2,*

¹浙江大学环境与资源学院资源科学系，浙江杭州 310058；²中国科学院城市环境研究所，福建厦门 310058；

收稿日期:2012-04-09 修回日期:2012-08-22 出版日期:2013-01-10 发布日期:2013-01-10
通讯作者: 姚槐应1，2,*
基金资助:
国家自然科学基金资助项目（41090283）；国家自然科学基金资助项目（31272256）。

Effects of Straw and Black Carbon Addition on CN Transformation and Microbial Metabolism Profile in Paddy Soil

WANG Juan ^1,2, ZHANG Lijun ¹,YAO Huaiying ^1,2,*

¹ Department of Resource Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; ² Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;

Received:2012-04-09 Revised:2012-08-22 Online:2013-01-10 Published:2013-01-10
Contact: YAO Huaiying1,2,*

摘要/Abstract

摘要： 通过向水稻土中添加秸秆和黑炭进行水稻盆栽实验（秸秆的添加量为2 g/kg和10 g/kg，黑炭的添加量为5 g/kg和25 g/kg），分别在孕穗期和成熟期取样研究土壤碳氮转化及微生物代谢剖面的变化。对土壤有机碳、全氮、铵氮等含量的测定结果显示，秸秆和黑炭均能于一定程度上促进土壤碳氮转化，提高水稻产量；Microresp方法检测的微生物代谢图谱表明，秸秆和黑炭的添加量越大，对微生物的代谢影响越大。造成这些差异的主要原因是添加秸秆和黑炭后微生物对果糖、丙氨酸、乙酰葡萄糖胺和赖氨酸盐酸盐的利用率上升。另外，秸秆对微生物碳和净碳矿化速率的影响显著高于黑炭，而黑炭对水稻产量和土壤固碳的影响更大。

关键词: 秸秆, 黑炭, 产量, Mircroresp

Abstract: The effects of straw and black carbon addition to paddy soil on carbon and nitrogen transformation were studied in a pot experiment. At booting and mature stages, soil CN transformation and microbial metabolism profile were analyzed. According to the results of organic carbon, total nitrogen, ammonium concentration, both straw and black carbon addition could promote CN transformation of paddy soil, thereby enhancing the yield of rice grain to some extent. The results of microbial metabolism identified by Microresp suggested that rising concentrations of straw and carbon had an increasing influence on microbial metabolism. The main reason behind the difference was the higher utilization of fructose, alanine, acetyl glucosamine and lysineHCl after the soil was amended with straw and black carbon. The effect of straw addition on microbial biomass carbon and net carbon mineralization was significantly higher than that of black carbon. Conversely, black carbon had higher effects on the yield of rice grain and soil carbon sequestration.

Key words: rice straw, black carbon, yield, Microresp

中图分类号:

王娟1，2,张丽君1,姚槐应1，2,* . 添加秸秆和黑炭对水稻土碳氮转化及土壤微生物代谢图谱的影响[J]. 中国水稻科学, 2013, 27(1): 97-104.

WANG Juan1,2, ZHANG Lijun1,YAO Huaiying1,2,*. Effects of Straw and Black Carbon Addition on CN Transformation and Microbial Metabolism Profile in Paddy Soil[J]. Chinese Journal of Rice Science, 2013, 27(1): 97-104.

参考文献

\[1\]Wang Y J, Bi Y Y, Gao C Y．The Assessment and utilization of straw resources in China．Arg Sci China，2010, 9(12): 18071815.

\[2\]邱孝煊, 蔡元呈, 林勇, 等．稻草还田对红壤性水稻土肥力的影响．中国农学通报, 2006, 22（1）: 188190.

\[3\]王玄德, 石孝均, 宋光煜．长期稻草还田对紫色水稻土肥力和生产力的影响．植物营养与肥料学报, 2005, 11（3）: 302307.

\[4\]Knoblauch C, Maarifat A A, Pfeiffer E M, et al． Degradability of black carbon and its impact on trace gas fluxes and carbon turnover in paddy soils．Soil Bio Biochem, 2010, 43(9): 17681778.

\[5\]Liu C, Wang K, Meng S, et al．Effects of irrigation, fertilization and crop straw management on nitrous oxide and nitric oxide emissions from a wheatmaize rotation field in northern China．Agr Ecosyst Environ, 2011, 140(1/2): 226233.

\[6\]Karhu K, Mattila T, Bergstrom I, et al．Biochar addition to agricultural soil increased CH4 uptake and water holding capacity-Results from a shortterm pilot field study．Agr Ecosyst Environ, 2011, 140(1/2): 309313.

\[7\]Peng X, Ye L, Wang C, et al．Temperature and durationdependent rice strawderived biochar: Characteristics and its effects on soil properties of an Ultisol in southern China．Soil Till Res, 2011, 111(1): 159166.

\[8\]Laird D A, Fleming P, Davis DD, et al． Impact of biochar amendments on the quality of a typical Midwestern agricultural soil．Geoderma, 2010, 158(3/4): 443449.

\[9\]Major J, Rondon M, Molina D, et al．Maize yield and nutrition during 4 years after biochar application to a Colombian savanna oxisol．Plant Soil, 2010, 333(1/2): 117128..

\[10\]Vaccari F, Baronti S, Lugato E, et al．Biochar as a strategy to sequester carbon and increase yield in durum wheat．Eur J Agron, 2011, 34(4): 231238.

\[11\]Beesley L, MorenoJiménez E, GomezEyles J L．Effects of biochar and greenwaste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multielement polluted soil. Environ Pollut, 2010, 158(6): 22822287.

\[12\]GomezEyles J L, Sizmur T, Collins C D, et al．Effects of biochar and the earthworm Eisenia fetida on the bioavailability of polycyclic aromatic hydrocarbons and potentially toxic elements．Environ Pollut, 2010, 159(2): 616622.

\[13\]谭周进, 李倩, 陈冬林, 等．稻草还田对晚稻土微生物及酶活性的影响．生态学报, 2006, 26（10）: 33853392.

\[14\]鲁如坤．土壤农业化学与分析方法．北京: 中国农业科技出版社, 2000.

\[15\]姚槐应, 黄昌勇．土壤微生物生态学及其实验技术．北京: 科学出版社, 2006: 147148.

\[16\]Campbell C D, Chapman S J, Cameron C M, et al．A rapid microtiter plate method to measure carbon dioxide evolved from carbon substrate amendments so as to determine the physiological profiles of soil microbial communities by using whole soil．Appl Environ Microb, 2003, 69(6): 35933599.

\[17\]Xu M, Lou Y, Sun X, et al．Soil organic carbon active fractions as early indicators for total carbon change under straw incorporation. Biol Fert Soils, 2011, 47(7): 745752.

\[18\]Vaccari F, Baronti S, Lugato E, et al. Biochar as a strategy to sequester carbon and increase yield in durum wheat．Eur J Agron, 2011, 34(4): 231238.

\[19\]肖小平, 汤海涛, 纪雄辉．稻草还田模式对稻田土壤速效氮、钾含量及晚稻生长的影响．作物学报, 2008, 34（8）: 14641469.

\[20\]彭英湘, 王凯荣, 谢小立, 等．水肥条件与稻草还田对土壤供氮及水稻产量的影响.中国土壤与肥料, 2007（4）: 4043.

\[21\]吴家梅, 彭华, 纪雄辉, 等．稻草还田方式对双季稻田耕层土壤有机碳积累的影响．生态环境学报, 2010, 19（10）: 2360 236.

\[22\]Malhi S, Nyborg M, Solberg E, et al．Improving crop yield and N uptake with longterm straw retention in two contrasting soil types．Field Crop Res, 2011, 124(3): 378391.

\[23\]Liang B, Yang X, He X, et al．Effects of 17year fertilization on soil microbial biomass C and N and soluble organic C and N in loessial soil during maize growth．Biol Fert Soils, 2011, 47(2): 121128.

\[24\]Aciego P J, Brookes P．Substrate inputs and pH as factors controlling microbial biomass, activity and community structure in an arable soil. Soil Bio Biochem, 2009, 41(7): 13961405.

\[25\]Dilly O, Bloem J, Vos A, et al．Bacterial diversity in agricultural soils during litter decomposition．Appl Environ Microb, 2004, 70(1): 468474.

\[26\]周文新, 陈冬林, 卜毓坚, 等．稻草还田对土壤微生物群落功能多样性的影响．环境科学学报, 2008, 28（2） : 3263305.

\[27\]Wu M, Qin H, Chen Z, et al．Effect of longterm fertilization on bacterial composition in rice paddy soil．Biol Fert Soils, 2011, 47(7): 397405.

\[28\]Zavalloni C, Alberti G, Biasiol S, et al．Microbial mineralization of biochar and wheat straw mixture in soil: A shortterm study．Appl Soil Ecol, 2011, 50: 4551.

\[29\]Jones D, Murphy D, Khalid M, et al．Shortterm biocharinduced increase in soil CO2 release is both biotically and abiotically mediated．Soil Bio Biochem, 2011, 43(8): 17231731.

\[30\]Keith A, Singh B, Singh B P．Interactive priming of biochar and labile organic matter mineralization in a smectiterich soil．Environ Sci Technol, 2011, 45(22): 96119618.

\[31\]Cross A, Sohi S P．The priming potential of biochar products in relation to labile carbon contents and soil organic matter status．Soil Bio Biochem, 2011, 43(10): 21272134.

\[32\]De Troyer I, Amery F, van Moorleghem C, et al．Tracing the source and fate of dissolved organic matter in soil after incorporation of a 13C labelled residue: A batch incubation study．Soil Bio Biochem, 2010, 43(3): 513519.

\[33\]Chapman S J, Campbell C D, Artz R R E．Assessing CLPPs using MicroRespTM．J Soil Sedimen, 2007, 7(6): 406410.

[1]	黄亚茹, 徐鹏, 王乐乐, 贺一哲, 王辉, 柯健, 何海兵, 武立权, 尤翠翠. 外源海藻糖对粳稻品系W1844籽粒灌浆特性及产量形成的影响[J]. 中国水稻科学, 2023, 37(4): 379-391.
[2]	高欠清, 任孝俭, 翟中兵, 郑普兵, 吴源芬, 崔克辉. 头季穗肥和促芽肥对再生稻再生芽生长及产量形成的影响[J]. 中国水稻科学, 2023, 37(4): 405-414.
[3]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[4]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[5]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[6]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[7]	裴峰, 王广达, 高鹏, 冯志明, 胡珂鸣, 陈宗祥, 陈红旗, 崔傲, 左示敏. 敲除OsNramp5基因创制低镉优质粳稻新材料的应用评价[J]. 中国水稻科学, 2023, 37(1): 16-28.
[8]	张露, 梁青铎, 吴龙龙, 黄晶, 田仓, 张均华, 曹小闯, 朱春权, 孔亚丽, 金千瑜, 朱练峰. 减氮和增氧灌溉对水稻产量和氮素利用的影响[J]. 中国水稻科学, 2023, 37(1): 78-88.
[9]	王颖姮, 陈丽娟, 崔丽丽, 詹生威, 宋煜, 陈世安, 解振兴, 姜照伟, 吴方喜, 卓传营, 蔡秋华, 谢华安, 张建福. 施氮量对优质稻“福香占”光合特性、产量及品质的影响[J]. 中国水稻科学, 2023, 37(1): 89-101.
[10]	任维晨, 常庆霞, 张亚军, 朱宽宇, 王志琴, 杨建昌. 不同氮利用率粳稻品种的碳氮积累与转运特征及其生理机制[J]. 中国水稻科学, 2022, 36(6): 586-600.
[11]	王敏羽, 戴志刚, 余德芳, 王向平, 关绍华, 邵远刚, 张家学, 李小坤. “水稻-再生稻”种植模式专用肥轻简施用对产量、肥料利用率及经济效益的影响[J]. 中国水稻科学, 2022, 36(5): 531-542.
[12]	曾文静, 邱岚英, 陈俊杰, 钱浩宇, 张楠, 丁艳锋, 江瑜. 秸秆还田下大气CO₂浓度升高对水稻生长和CH₄排放的影响[J]. 中国水稻科学, 2022, 36(5): 543-550.
[13]	张宇杰, 王志强, 马鹏, 杨志远, 孙永健, 马均. 麦秆还田下水氮耦合对水稻氮素吸收利用及产量的影响[J]. 中国水稻科学, 2022, 36(4): 388-398.
[14]	陈云, 刘昆, 李婷婷, 李思宇, 李国明, 张伟杨, 张耗, 顾骏飞, 刘立军, 杨建昌. 结实期干湿交替灌溉对水稻根系、产量和土壤的影响[J]. 中国水稻科学, 2022, 36(3): 269-277.
[15]	张小祥, 邵士梅, 赵步洪, 张耗, 季红娟, 肖宁, 潘存红, 李育红, 吴云雨, 蔡跃, 刘建菊, 吉春明, 张秀琴, 刘广青, 周长海, 黄年生, 李爱宏. 氮肥减施模式对不同穗型迟熟中粳水稻产量及氮素吸收利用的影响[J]. 中国水稻科学, 2022, 36(3): 278-294.

添加秸秆和黑炭对水稻土碳氮转化及土壤微生物代谢图谱的影响

Effects of Straw and Black Carbon Addition on CN Transformation and Microbial Metabolism Profile in Paddy Soil

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics